Florence Farmborough was born in Steeple Claydon, Buckinghamshire, on 15th April 1887. She was named after Florence Nightingale, who lived nearby. She was educated at home by a governess before attending St. Thorolds in London.
Florence moved to Russia in 1908 where she found work as an English teacher. On the outbreak of the First World War, Farmborough immediately offered her services as a nursing sister at the hospital established by Princess Golitsin in Moscow. Later she accompanied Russian troops in Poland, Austria and Romania.